1Centre for Myopia Research, School of Optometry,The Hong Kong Polytechnic University, 2Centre for Eye and Vision Research (CEVR), 3Research Centre for SHARP Vision (RCSV),The Hong Kong Polytechnic University, 4Research Centre for Chinese Medicine Innovation (RCMI),The Hong Kong Polytechnic University
Summary
This protocol describes a method for collecting tear samples using Schirmer strips and an integrated quantitative workflow for discovering non-invasive tear protein biomarkers. The suspension trapping sample preparation workflow enables fast and robust tear sample preparation and mass spectrometric analysis, resulting in higher peptide recovery yields and protein identification than standard in-solution procedures.
