Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:17Introduction
- 01:10Dissection of the Bulge from Adult Mouse Whisker Follicles
- 04:34Culture of Bulge Explants
- 07:18Conclusion
Here we show our method to isolate mouse epidermal neural crest stem cells (EPI-NCSC). Technique involves micro-dissecting whisker follicles, isolating the bulge and placeing it into tissue culture. EPI-NCSC start to emigrate from bulge explants onto the substratum within 3 - 4 days.
Tags
Mouse Epidermal Neural Crest Stem CellEPI-NCSCEmbryonic Neural CrestHair FolliclesMultipotent Stem CellsDifferentiated Cell TypesNeuronsNerve Supporting CellsSmooth Muscle CellsBone/cartilage CellsMelanocytesHairy SkinPure Population Of Stem CellsProtocolWhisker FolliclesWhisker PadCollagen CapsuleCulture PlateEmigration
