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Flow Cytometry-Based Assays for Microglial Engulfment of Synaptic Materials
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Author Spotlight: Advancing Understanding Through Technological Innovations in Psychoneuroimmunology
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Quantification of Microglial Engulfment of Synaptic Material Using Flow Cytometry

Author Spotlight: Advancing Understanding Through Technological Innovations in Psychoneuroimmunology

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01:38 min

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May 31, 2024

DOI:

10.3791/66639-v

DOI:
10.3791/66639-v

•

01:38 min
May 31, 2024

•

8 Views
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1Psychoneuroimmunology,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, 2Experimental Ophthalmology,Charité Universitätsmedizin Berlin

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MicrogliaSynaptic MaterialFlow CytometrySynaptic RefinementImmunohistochemistryPHrodo RedSynaptosomesCell IsolationIn VivoIn VitroHigh-throughputQuantification

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Ugursu, B., Wolf, S. A. Quantification of Microglial Engulfment of Synaptic Material Using Flow Cytometry. J. Vis. Exp. (207), e66639, doi:10.3791/66639 (2024).

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