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Enhancing Cryo-EM Map Quality with CryoSieve Particle Selection

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Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency

Journal JoVE
Biologie

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Journal JoVE Biologie

Enhancing Density Maps by Removing the Majority of Particles in Single Particle Cryogenic Electron Microscopy Final Stacks

Author Spotlight: Optimizing Cryo-EM Analysis with CryoSieve for Enhanced Particle Selection Efficiency

Enhancing Cryo-EM Map Quality with CryoSieve Particle Selection

DOI:

10.3791/66617-v

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01:45 min

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May 10, 2024

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Mengjia Cai*¹, Jianying Zhu*², Qi Zhang*^3,4,5,6, Yu Xu, Zuoqiang Shi⁸, Chenglong Bao^8,9, Mingxu Hu^5,6

¹Institute of Bio-Architecture and Bio-Interactions (IBABI),Shenzhen Medical Academy of Research and Translation, ²Yau Mathematical Sciences Center, ³Key Laboratory of Protein Science (Tsinghua University),Ministry of Education, ⁴School of Life Science,Tsinghua University, ⁵Beijing Advanced Innovation Center for Structural Biology, ⁶Beijing Frontier Research Center for Biological Structure, ⁷Department of Mathematical Sciences,Tsinghua University, ⁸Yanqi Lake Beijing Institute of Mathematical Sciences and Applications, ⁹State Key Laboratory of Membrane Biology, School of Life Science

Tags

Cryogenic Electron Microscopy Cryo-EM Single-particle Analysis SPA Density Maps Particle Selection CryoSieve High-resolution Structural Examination Molecular Insights X-ray Crystallography

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