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Rat "Brain Milking" for Neural Stem and Oligodendrocyte Progenitor Cell Isolation
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Author Spotlight: Collecting Neural Stem and Progenitor Cells from Live Animals Using a Novel Brain Milking Protocol
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Neurosciences
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Journal JoVE Neurosciences
The “Brain Milking” Method for the Isolation of Neural Stem Cells and Oligodendrocyte Progenitor Cells from Live Rats

Author Spotlight: Collecting Neural Stem and Progenitor Cells from Live Animals Using a Novel Brain Milking Protocol

1,220 Views

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01:42 min

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February 09, 2024

DOI:

10.3791/65308-v

DOI:
10.3791/65308-v

•

01:42 min
February 09, 2024

•

7 Views
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1Laboratory of Developmental Biology, Department of Biology,University of Patras, 2Wellcome Trust-MRC Cambridge Stem Cell Institute,University of Cambridge, 3Altos Labs,Cambridge Institute of Science

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Neural Stem CellsOligodendrocyte Progenitor CellsBrain MilkingLive RatsRegenerationQuiescenceActivationDifferentiationNeurogenesisGliogenesisSubependymal ZoneVentricular-subventricular ZoneCentral Nervous System

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Dimitrakopoulos, D., Dimitriou, C., McClenahan, F., Franklin, R. J. M., Kazanis, I. The "Brain Milking" Method for the Isolation of Neural Stem Cells and Oligodendrocyte Progenitor Cells from Live Rats. J. Vis. Exp. (204), e65308, doi:10.3791/65308 (2024).

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