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Whole Neonatal Cochlear Explants as an In vitro Model
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Neurosciences
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Journal JoVE Neurosciences
Whole Neonatal Cochlear Explants as an In vitro Model

Whole Neonatal Cochlear Explants as an In vitro Model

DOI:
10.3791/65160-v

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07:12 min

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July 28, 2023

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, , ,
1Department of Biomedicine,University of Basel Hospital, 2Department of Otorhinolaryngology, Head and Neck Surgery,University of Basel Hospital

Chapitres

  • 00:04Introduction
  • 01:13Isolation of Cochlea, Corti, and Cochlear Explants
  • 03:31Cochlear Explants Culturing
  • 05:04Results: Identification of Corti and Cochlear Explants
  • 06:30Conclusion

Summary

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The current protocol updates previous protocols and incorporates relatively straightforward approaches for culturing high-quality cochlear explants. This provides reliable data acquisition and high-resolution imaging in live and fixed cells. This protocol supports the ongoing trend of studying inner ear cells.

Tags

Neonatal Cochlear ExplantIn Vitro ModelSensorineural Hearing LossOtotoxic ModelStructural AnalysisSignaling PathwaysDrug ScreeningEmbryonic DevelopmentCochlear DissectionCochleaSpiral GanglionSpiral LigamentStria VascularisReissner’s Membrane

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