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Development of Leishmania Species Strains with Constitutive Expression of eGFP
Journal JoVE
Génétique
Author Produced
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Journal JoVE Génétique
Development of Leishmania Species Strains with Constitutive Expression of eGFP

Development of Leishmania Species Strains with Constitutive Expression of eGFP

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10:03 min

April 21, 2023

DOI:

10:03 min
April 21, 2023

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Summary

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Here, we describe the methodology used for generating L. panamensis and L. donovani strains expressing the gene for eGFP as a stable integrated transgene using the pLEXSY system. Transfected parasites were cloned by limiting dilution, and clones with the highest fluorescence intensity in both species were selected for further use in drug screening assays.

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