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Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
DOI:

09:02 min

September 22, 2023

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Chapitres

  • 00:04Introduction
  • 00:57Infection of Nasal Air‐Liquid Interface (ALI) Cultures
  • 02:53Collection and Quantification of the Shed Virus in Apical Surface Liquid (ASL)
  • 03:49Transepithelial Electrical Resistance (TEER) Measurement
  • 05:25Measuring Cytotoxicity by Lactate Dehydrogenase (LDH) Assay
  • 06:18Results: Characterizing Viral Replication, Host Cell Tropism, and Virus‐Induced Cytotoxicity in Nasal ALI Cultures
  • 08:11Conclusion

Summary

Traduction automatique

The nasal epithelium is the primary barrier site encountered by all respiratory pathogens. Here, we outline methods to use primary nasal epithelial cells grown as air-liquid interface (ALI) cultures to characterize human coronavirus-host interactions in a physiologically relevant system.

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