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Edição Gênica de Células B Primárias de Macaco Rhesus
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February 10, 2023
DOI:
1Laboratory of Molecular Immunology,The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 3Laboratory of Retrovirology,The Rockefeller University, 4Howard Hughes Medical Institute,The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine,King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center,Wistar Institute
Summary
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Apresentamos um método de cultura e edição gênica de células B primárias de macaco rhesus usando CRISPR/Cas9 e vírus adenoassociado recombinante sorotipo 6 para o estudo de terapias com células B.
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Hartweger, H., Gautam, R., Nishimura, Y., Schmidt, F., Yao, K., Escolano, A., Jankovic, M., Martin, M. A., Nussenzweig, M. C. Gene Editing of Primary Rhesus Macaque B Cells. J. Vis. Exp. (192), e64858, doi:10.3791/64858 (2023).
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