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In vivo Calciumbilleddannelse af neuronale ensembler i netværk af primære sensoriske neuroner i intakte dorsale rodganglier
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Journal JoVE Neurosciences
In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia

In vivo Calciumbilleddannelse af neuronale ensembler i netværk af primære sensoriske neuroner i intakte dorsale rodganglier

2,234 Views

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09:07 min

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February 10, 2023

DOI:

10.3791/64826-v

DOI:
10.3791/64826-v

•

09:07 min
February 10, 2023

•

19 Views
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1Department of Oral & Maxillofacial Surgery, School of Dentistry,University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences,University of Texas Health Science Center at San Antonio

Summary

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Denne protokol beskriver den kirurgiske eksponering af dorsalrodsganglion (DRG) efterfulgt af GCaMP3 (genetisk kodet Ca2+ indikator; Grønt fluorescerende protein-calmodulin-M13-protein 3) Ca2+ billeddannelse af neuronale ensembler ved hjælp af Pirt-GCaMP3-mus, mens der påføres en række stimuli på den ipsilaterale bagpote.

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Cite this Article

Shannonhouse, J., Gomez, R., Son, H., Zhang, Y., Kim, Y. S. In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia. J. Vis. Exp. (192), e64826, doi:10.3791/64826 (2023).

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