Journal
/
Neurosciences
/
In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia

In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia

2,259 Views

•

09:07 min

•

February 10, 2023

DOI:

10.3791/64826-v

DOI:
10.3791/64826-v

•

09:07 min
February 10, 2023

•

19 Views
, , , ,
1Department of Oral & Maxillofacial Surgery, School of Dentistry,University of Texas Health Science Center at San Antonio, 2Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences,University of Texas Health Science Center at San Antonio

Summary

Automatically generated

This protocol describes the surgical exposure of the dorsal root ganglion (DRG) followed by GCaMP3 (genetically-encoded Ca2+ indicator; Green Fluorescent Protein-Calmodulin-M13 Protein 3) Ca2+ imaging of the neuronal ensembles using Pirt-GCaMP3 mice while applying a variety of stimuli to the ipsilateral hind paw.

Explore More Videos

In Vivo Calcium ImagingNeuronal EnsemblesPrimary Sensory NeuronsDorsal Root GangliaPeripheral AllodyniaPain HypersensitivityTrigeminal GangliaGeniculate GangliaGenetically Encoded SensorsVoltageCell Signaling MoleculesCyclic AMPSurgical ProcedureLumbar EnlargementTransverse ProcessL5 DRGIsoflurane Anesthesia

Read Article

Cite this Article

Shannonhouse, J., Gomez, R., Son, H., Zhang, Y., Kim, Y. S. In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia. J. Vis. Exp. (192), e64826, doi:10.3791/64826 (2023).

Download .ris file

Copy

Share Video

.

Copy