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Um Pipeline para Caracterizar Defeitos Cardíacos Estruturais em Rato Fetal
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Biologie du développement
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Journal JoVE Biologie du développement
A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

Um Pipeline para Caracterizar Defeitos Cardíacos Estruturais em Rato Fetal

1,625 Views

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08:19 min

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December 16, 2022

DOI:

10.3791/64582-v

DOI:
10.3791/64582-v

•

08:19 min
December 16, 2022

•

1 Views
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1Department of Developmental Biology,University of Pittsburgh, 2Department of Biological Sciences,University of Pittsburgh, 3Département de Biologie,École Normale Supérieure de Lyon, 4Department of Critical Care Medicine,University of Pittsburgh

Summary

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Este artigo detalha os métodos diagnósticos de cardiopatia congênita murina (DCC) usando ecocardiografia fetal, necropsia e captura de imagem de fluorescência episcópica (EFIC) usando microscopia confocal episcópica (ECM) seguida de reconstrução tridimensional (3D).

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Fetal MouseCongenital Heart DefectsEchocardiographyNecropsyConfocal MicroscopyAnatomical ChangesStructural Birth DefectsUltrasoundColor DopplerSample PreparationLaser Confocal ImagingImage Acquisition

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Cite this Article

Guzman-Moreno, C., Zhang, P., Phillips, O. R., Block, M., Glennon, B. J., Holbrook, M., Weigand, L., Lo, C. W., Lin, J. I. A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse. J. Vis. Exp. (190), e64582, doi:10.3791/64582 (2022).

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