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Developmental Biology

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JoVE Journal Developmental Biology
An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)
 

An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)

Article DOI: 10.3791/64445-v • 06:26 min • September 7th, 2022
September 7th, 2022 •
1,2, 1,2,3, 1
1Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 2College of Life Science, University of Chinese Academy of Sciences, 3Innovative Academy of Seed Design, Chinese Academy of Sciences

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résumé
Automatic Translation

The tomato seed is an important model for studying genetics and developmental biology during plant reproduction. This protocol is useful for clearing tomato seeds at different developmental stages to observe the finer embryonic structure.

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Keywords: Seed Development Tomato Solanum Lycopersicum Clearing Protocol Embryo Observation Chemical Preparation Fruit Harvesting Seed Collection Seed Fixation Seed Dehydration Clearing Solution Microscopy Disinfectant Solution Seed Mucilage Removal Seed Washing
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