Journal
/
Biologie du développement
/
Ablação a Laser Multifóton de Centros Organizadores de Microtúbulos Citoplasmáticos em Oócitos de Camundongos
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Ablação a Laser Multifóton de Centros Organizadores de Microtúbulos Citoplasmáticos em Oócitos de Camundongos

1,327 Views

•

08:24 min

•

November 11, 2022

DOI:

10.3791/64439-v

DOI:
10.3791/64439-v

•

08:24 min
November 11, 2022

•

5 Views
, ,
1Animal Sciences Research Center,University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core,University of Missouri

Summary

Automatically generated

Apresenta-se um protocolo otimizado que permite a depleção de centros organizadores de microtúbulos citoplasmáticos em ovócitos de camundongos durante a metáfase I utilizando um laser de femtossegundos no infravermelho próximo.

Explore More Videos

Multi-photon Laser AblationCytoplasmic Microtubule Organizing CentersMouse OocytesSpindle PositioningMeiosisAneuploidyMTOCsPolar MTOCsMcMTOCsTargeted AblationLive CellPhototoxicityMechanical PerturbationCell Viability

Read Article

Cite this Article

Londoño-Vásquez, D., Jurkevich, A., Balboula, A. Z. Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes. J. Vis. Exp. (189), e64439, doi:10.3791/64439 (2022).

Download .ris file

Copy

Share Video

.

Copy