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Multi-foton laserablatie van cytoplasmatische microtubule organiserende centra in muizenoöcyten
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
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Multi-foton laserablatie van cytoplasmatische microtubule organiserende centra in muizenoöcyten

1,327 Views

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08:24 min

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November 11, 2022

DOI:

10.3791/64439-v

DOI:
10.3791/64439-v

•

08:24 min
November 11, 2022

•

5 Views
, ,
1Animal Sciences Research Center,University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core,University of Missouri

Summary

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Er wordt een geoptimaliseerd protocol gepresenteerd dat de uitputting van cytoplasmatische microtubuli organiserende centra in muizenoöcyten tijdens metafase I mogelijk maakt met behulp van een nabij-infrarode femtosecondelaser.

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Cite this Article

Londoño-Vásquez, D., Jurkevich, A., Balboula, A. Z. Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes. J. Vis. Exp. (189), e64439, doi:10.3791/64439 (2022).

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