Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
1,934 Views
•
10:16 min
•
January 20, 2023
DOI:
Summary
Automatically generated
This protocol provides a rapid and size-specific isolation method for small extracellular vesicles by optimizing the size of the air spray nozzle, sheath fluid pressure, sample flow pressure, voltage, gain, and triggering threshold parameters.
Explore More Videos
Flow CytometryExtracellular VesiclesSmall Extracellular VesiclesSize-specific SortingHigh-throughput IsolationPurificationFlow Cytometric Sorting ParametersForward ScatterSize ScatterStartup ProcedureLaser PowerSheath FluidSample FlowStream AlignmentLaser InterceptNozzle AlignmentFine Laser AlignmentRainbow Fluorescent ParticlesQuality ControlIntelliSort
Read Article
Cite this Article
Song, X., Shen, H., Li, Y., Xing, Y., Wang, J., Guo, C., Huang, Y., Chen, J. Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles. J. Vis. Exp. (191), e64360, doi:10.3791/64360 (2023).
Copy