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Hybrid Cell Analysis System to Assess Structural and Contractile Changes of Human iPSC-Derived Cardiomyocytes for Preclinical Cardiac Risk Evaluation
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Journal JoVE Bio-ingénierie
Hybrid Cell Analysis System to Assess Structural and Contractile Changes of Human iPSC-Derived Cardiomyocytes for Preclinical Cardiac Risk Evaluation

Hybrid Cell Analysis System to Assess Structural and Contractile Changes of Human iPSC-Derived Cardiomyocytes for Preclinical Cardiac Risk Evaluation

DOI:
10.3791/64283-v

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08:03 min

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October 20, 2022

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, , , , , , , ,
1innoVitro GmbH, 2Nanion Technologies GmbH

Chapitres

  • 00:04Introduction
  • 00:53Plate Coating
  • 01:58Seeding of Human iPSC‐Derived Cardiomyocytes into Flexible 96‐Well Plates (Day 0)
  • 02:56Medium Exchange of Flexible 96‐Well Plates (Day 1)
  • 03:53Final Medium Exchange Before Compound Addition (Day 5–7)
  • 04:21Compound Addition and Data Recording (Day 5–7)
  • 05:47Results: Contractility Assessment, Base Impedance, and EFP Recordings of Human iPSC‐Derived Cardiomyocytes After Drug Treatment
  • 07:35Conclusion

Summary

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The analysis of changes in contractile function and cellular integrity of human iPSC-derived cardiomyocytes is of immense importance for nonclinical drug development. A hybrid 96-well cell analysis system addresses both parameters in a real-time and physiological manner for reliable, human-relevant results, necessary for a safe transition into clinical stages.

Tags

Keywords: Hybrid Cell Analysis SystemIPSC-derived CardiomyocytesPreclinical Cardiac Risk EvaluationElectrophysiological AssessmentStructural AnalysisContractile PropertiesDrug DiscoveryHigh-throughput TestingCell SeedingEHS Gel CoatingCell CultureCardiomyocyte Maintenance Medium

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