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成年小鼠大脑中诱导荧光标记干细胞的谱系示踪
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Neurosciences
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Journal JoVE Neurosciences
Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
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成年小鼠大脑中诱导荧光标记干细胞的谱系示踪

2,594 Views

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09:44 min

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May 20, 2022

DOI:

10.3791/63998-v

DOI:
10.3791/63998-v

•

09:44 min
May 20, 2022

•

11 Views
, , ,
1Graduate School of Biomedical Science and Engineering,University of Maine, 2Department of Neurosurgery,Ohio State University Wexner Medical Center, 3Boston Children’s Hospital,Harvard Medical School

Summary

Automatically generated

使用诱导的转基因谱系追踪小鼠系，用荧光团永久标记干细胞及其后代的能力允许对 体内的活化，增殖，迁移和/或分化进行空间和时间分析。谱系追踪可以揭示有关谱系承诺、对干预的反应和多能性的新信息。

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Cite this Article

Jensen, G. S., Willows, J. W., Breault, D. T., Townsend, K. L. Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain. J. Vis. Exp. (183), e63998, doi:10.3791/63998 (2022).

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