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Bioengineering

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JoVE Journal Bioengineering
Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
 

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Article DOI: 10.3791/63988-v • 09:30 min • June 2nd, 2022
June 2nd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Department of Biomedical Engineering, Eindhoven University of Technology, 2Institute for Complex Molecular Systems, Eindhoven University of Technology

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résumé
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Traditionally, cell culture is performed on planar substrates that poorly mimic the natural environment of cells in vivo. Here we describe a method to produce cell culture substrates with physiologically relevant curved geometries and micropatterned extracellular proteins, allowing systematic investigations into cellular sensing of these extracellular cues.

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UV-photopatterning Multicue Cellular Microenvironments Three-dimensional Cell Culture Substrates Microenvironmental Factors In Vitro Platforms Cell Behavior Cell Culture Materials Contact Guidance Patterns Cellular Readouts Proteins Glass Mold Femtosecond Laser Direct Right Technique Polydimethylsiloxane Pre Polymer Vacuum Desiccator Vacuum Pump Curing PDMS Chip
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