Polyp bail-out is a process induced by acute stress, in which coral polyps digest the tissue connecting them to their colony and detach from it to live as individuals. The present protocol describes how to induce coral micropropagation by bail-out using hypersaline or calcium-free seawater treatments.
Cardoso, P. M., Alsaggaf, A. A., Villela, H. M., Peixoto, R. S. Inducing Polyp Bail-out in Coral Colonies to Obtain Individualized Micropropagates for Laboratory Experimental Use. J. Vis. Exp. (182), e63840, doi:10.3791/63840 (2022).