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Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons
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Journal JoVE Neurosciences
Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

DOI:
10.3791/63800-v

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09:40 min

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June 30, 2022

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, , , , ,
1Department of Pharmacology and Physiology,Université de Montréal

Chapitres

  • 00:05Introduction
  • 00:47Natural Killer (NK) Cell Isolation, Culture, and Stimulation
  • 02:25Dorsal Root Ganglia (DRG) Neuron Isolation and Culture
  • 06:13Co-Culture and Flow Cytometry
  • 07:58Results: Interplay Between Natural Killer Cells and Nociceptor Neurons
  • 08:41Conclusion

Summary

Traduction automatique

Traduction automatique

Nociceptor neurons and NK cells actively interact in an inflammatory context. A co-culture approach enables studying this interplay.

Tags

Natural Killer CellsNociceptor NeuronsCo-cultureAnti-tumor FunctionNK Cell IsolationMouse SpleenRPMI 1640 MediumIL-2IL-15LamininLumbosacral JointSacrumLumbar SpineAtlantooccipital Joint

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