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Developmental Biology

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JoVE Journal Developmental Biology
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
 

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Article DOI: 10.3791/63388-v • 12:09 min • August 10th, 2022
August 10th, 2022 •
*1, *1, *1, 1,3, 1,3, 2, 1, 1
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), 2Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna
* These authors contributed equally

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résumé
Automatic Translation

A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells.

Tags

Human Blastoids Blastocyst Development Implantation Research Models Scientific Progress Medical Progress Cellular Determination Morphogenesis Therapeutic Targets Pre-clinical Modeling In Vitro Fertilization Medium Non-hormonal Contraceptives PXGL Media N2B27 Basal Media Washing Buffer PBS Aggregation Media MEF Exclusion Gelatin Coated Plate Cell Detachment Solution
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