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JoVE Journal Developmental Biology
将人 iPSC 稳健分化为纯脂肪细胞群以研究脂肪细胞相关疾病
 
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将人 iPSC 稳健分化为纯脂肪细胞群以研究脂肪细胞相关疾病

Article DOI: 10.3791/63311-v • 10:31 min • February 9th, 2022
February 9th, 2022 •
1,2, 1, 1,2
1Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), 2College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation

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该方案允许从诱导多能干细胞（iPSC）产生纯脂肪细胞群。维甲酸用于将iPSCs分化为间充质干细胞（MSC），用于产生脂肪细胞。然后，采用基于尼罗河红染色的分选方法获得纯脂肪细胞。

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本月在JoVE上，第180期，诱导多能干细胞，间充质干细胞，脂肪细胞分化，细胞分选
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