Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Maximilian Wulf; Katalin Barkovits-Boeddinghaus; Paula Sommer; Karin Schork; Martin Eisenacher; Peter Riederer; Manfred Gerlach; Steffen K&#246;sters; Britta Eggers; Katrin Marcus

doi:10.3791/63289

Journal

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Neurosciences

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Nöromelanin Granüllerinin Proteomik Profilinin LC-MS/MS Analizi için Lazer Mikrodiseksiyon Tabanlı Protokol

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Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

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Nöromelanin Granüllerinin Proteomik Profilinin LC-MS/MS Analizi için Lazer Mikrodiseksiyon Tabanlı Protokol

2,001 Views

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07:35 min

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December 16, 2021

DOI:

10.3791/63289-v

DOI:

10.3791/63289-v

•

07:35 min

December 16, 2021

•

32 Views

Maximilian Wulf^1,2, Katalin Barkovits-Boeddinghaus^1,2, Paula Sommer^1,2, Karin Schork^1,2, Martin Eisenacher^1,2, Peter Riederer^3,4, Manfred Gerlach⁵, Steffen Kösters^1,2, Britta Eggers*^1,2, Katrin Marcus*^1,2

¹Medizinisches Proteom-Center, Medical Faculty,Ruhr-University Bochum, ²Medical Proteome Analysis, Center for Proteindiagnostics (PRODI),Ruhr-University Bochum, ³Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy,University Hospital Wuerzburg, ⁴Psychiatry Department of Clinical Research,University of Southern Denmark Odense University Hospital, ⁵Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy,University Hospital of Wuerzburg, University of Wuerzburg

Summary

Burada, nöromelanin granüllerinin lazer mikrodiseksiyonu yoluyla insan post-mortem substantia nigra pars compacta dokusundan izole edilmesi için sağlam bir protokol sunulmuştur. Bu revize edilmiş ve optimize edilmiş protokol, numune toplama için gereken süreyi büyük ölçüde en aza indirir, gerekli numune miktarını azaltır ve LC-MS / MS analizi ile proteinlerin tanımlanmasını ve nicelleştirilmesini geliştirir.

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Laser Microdissection Proteomic Profile Neuromelanin Granules Mass Spectrometry Proteomic Analysis Disease Mechanisms Robo Stage Field Of View Analysis Laser Settings Sample Collection Triptic Digestion HPLC-MS Analysis Proteomic Raw Data Analysis

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Nöromelanin Granüllerinin Proteomik Profilinin LC-MS/MS Analizi için Lazer Mikrodiseksiyon Tabanlı Protokol

Summary

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