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Protocolo Baseado em Microdissecção a Laser para a Análise LC-MS/MS do Perfil Proteômico de Grânulos de Neuromelanina
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Journal JoVE Neurosciences
Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Protocolo Baseado em Microdissecção a Laser para a Análise LC-MS/MS do Perfil Proteômico de Grânulos de Neuromelanina

2,001 Views

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07:35 min

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December 16, 2021

DOI:

10.3791/63289-v

DOI:
10.3791/63289-v

•

07:35 min
December 16, 2021

•

32 Views
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1Medizinisches Proteom-Center, Medical Faculty,Ruhr-University Bochum, 2Medical Proteome Analysis, Center for Proteindiagnostics (PRODI),Ruhr-University Bochum, 3Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy,University Hospital Wuerzburg, 4Psychiatry Department of Clinical Research,University of Southern Denmark Odense University Hospital, 5Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy,University Hospital of Wuerzburg, University of Wuerzburg

Summary

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Um protocolo robusto é apresentado aqui para isolar grânulos de neuromelanina de tecido humano post-mortem substantia nigra pars compacta via microdissecção a laser. Este protocolo revisado e otimizado minimiza massivamente o tempo necessário para a coleta de amostras, reduz a quantidade de amostra necessária e melhora a identificação e quantificação de proteínas pela análise LC-MS/MS.

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Cite this Article

Wulf, M., Barkovits-Boeddinghaus, K., Sommer, P., Schork, K., Eisenacher, M., Riederer, P., Gerlach, M., Kösters, S., Eggers, B., Marcus, K. Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules. J. Vis. Exp. (178), e63289, doi:10.3791/63289 (2021).

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