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基于激光显微切割的协议，用于神经黑色素颗粒蛋白质组学谱的LC-MS / MS分析

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Neurosciences

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Journal JoVE Neurosciences

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

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基于激光显微切割的协议，用于神经黑色素颗粒蛋白质组学谱的LC-MS / MS分析

2,001 Views

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07:35 min

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December 16, 2021

DOI:

10.3791/63289-v

DOI:

10.3791/63289-v

•

07:35 min

December 16, 2021

•

32 Views

Maximilian Wulf^1,2, Katalin Barkovits-Boeddinghaus^1,2, Paula Sommer^1,2, Karin Schork^1,2, Martin Eisenacher^1,2, Peter Riederer^3,4, Manfred Gerlach⁵, Steffen Kösters^1,2, Britta Eggers*^1,2, Katrin Marcus*^1,2

¹Medizinisches Proteom-Center, Medical Faculty,Ruhr-University Bochum, ²Medical Proteome Analysis, Center for Proteindiagnostics (PRODI),Ruhr-University Bochum, ³Center of Mental Health; Clinic and Policlinic for Psychiatry, Psychosomatics and Psychotherapy,University Hospital Wuerzburg, ⁴Psychiatry Department of Clinical Research,University of Southern Denmark Odense University Hospital, ⁵Center of Mental Health, Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy,University Hospital of Wuerzburg, University of Wuerzburg

Summary

这里提出了一个强大的方案，用于通过激光显微切割从人死后黑质致密组织中分离神经黑色素颗粒。该修订和优化的方案大大减少了样品采集所需的时间，减少了所需的样品量，并通过LC-MS/MS分析增强了蛋白质的鉴定和定量。

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