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用于疫苗开发的无细胞规模生产和对来自 穆里达鲁姆衣原 体的重组主要外膜蛋白的佐剂添加
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
DOI:

12:53 min

March 16, 2022

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Chapitres

  • 00:04Introduction
  • 01:00Cell‐Free Production of MOMP‐tNLPs for Subunit Vaccine Formulations
  • 04:21MOMP‐tNLP Purification
  • 07:19Western and Dot Blots and Storage
  • 10:14Results: SDS‐PAGE Analysis
  • 11:46Conclusion

Summary

Traduction automatique

该协议描述了使用商业的,无细胞的蛋白质表达试剂盒来产生纳米盘中支持的膜蛋白,该膜蛋白可用作亚单位疫苗中的抗原。

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