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Generación de organoides cerebrales a partir de células madre pluripotentes inducidas en mini biorreactores caseros
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Generación de organoides cerebrales a partir de células madre pluripotentes inducidas en mini biorreactores caseros
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10:16 min
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December 11, 2021
DOI:
1Federal Research and Clinical Center of Physical-Chemical Medicine,Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics,Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine,Federal Medical Biological Agency, 4Department of Pathological Anatomy,North-Western State Medical University, 5Department of Biology,Lomonosov State University
Summary
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Aquí describimos un protocolo para generar organoides cerebrales a partir de células madre pluripotentes inducidas por humanos (iPSC). Para obtener organoides cerebrales en grandes cantidades y de alta calidad, utilizamos mini biorreactores caseros.
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Cite this Article
Eremeev, A., Belikova, L., Ruchko, E., Volovikov, E., Zubkova, O., Emelin, A., Deev, R., Lebedeva, O., Bogomazova, A., Lagarkova, M. Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors. J. Vis. Exp. (178), e62987, doi:10.3791/62987 (2021).
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