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基于时空波动光谱的运输亚细胞纳米结构的结构和动态性质

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

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基于时空波动光谱的运输亚细胞纳米结构的结构和动态性质

1,531 Views

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08:17 min

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August 16, 2021

DOI:

10.3791/62790-v

DOI:

10.3791/62790-v

•

08:17 min

August 16, 2021

•

2 Views

Gianmarco Ferri¹, Fabio Azzarello¹, Francesca D’Autilia², Francesco Cardarelli¹

¹NEST Laboratory,Scuola Normale Superiore, ²Center for Nanotechnology Innovation@NEST CNI@NEST

Summary

成像衍生的均方位移（iMSD）分析应用于大刺激小体，以突出它们在结构和动态特性方面的内在时间演化性质。然后将大孔蛋白酶体与胰岛素分泌颗粒（ISG）进行比较，作为具有时间不变平均结构/动态特性的亚细胞结构的参考。

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