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斑马鱼胚胎快速心脏动力学的光片显微镜
Journal JoVE
Biologie
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Journal JoVE Biologie
Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos
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斑马鱼胚胎快速心脏动力学的光片显微镜

3,620 Views

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07:29 min

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August 13, 2021

DOI:

10.3791/62741-v

DOI:
10.3791/62741-v

•

07:29 min
August 13, 2021

•

11 Views
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1Morgridge Institute for Research, Madison, WI

Summary

Automatically generated

我们描述了用光片荧光显微镜 在体内 研究斑马鱼心脏的优化工具。具体而言，我们建议使用明亮的心脏转基因系，并提出新的温和嵌入和固定技术，以避免发育和心脏缺陷。还提供了适用于心脏成像的可能的数据采集和分析管道。

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Cite this Article

Schlaeppi, A., Graves, A., Weber, M., Huisken, J. Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos. J. Vis. Exp. (174), e62741, doi:10.3791/62741 (2021).

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