We describe optimized tools to study the zebrafish heart in vivo with light sheet fluorescence microscopy. Specifically, we suggest bright cardiac transgenic lines and present new gentle embedding and immobilization techniques that avoid developmental and heart defects. A possible data acquisition and analysis pipeline adapted to cardiac imaging is also provided.
Schlaeppi, A., Graves, A., Weber, M., Huisken, J. Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos. J. Vis. Exp. (174), e62741, doi:10.3791/62741 (2021).