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Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos

Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos

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07:29 min

August 13, 2021

DOI:

07:29 min
August 13, 2021

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Summary

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We describe optimized tools to study the zebrafish heart in vivo with light sheet fluorescence microscopy. Specifically, we suggest bright cardiac transgenic lines and present new gentle embedding and immobilization techniques that avoid developmental and heart defects. A possible data acquisition and analysis pipeline adapted to cardiac imaging is also provided.

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