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Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
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Biologie
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Journal JoVE Biologie
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

2,904 Views

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14:40 min

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March 05, 2022

DOI:

10.3791/62426-v

DOI:
10.3791/62426-v

•

14:40 min
March 05, 2022

•

4 Views
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1Institute of Microbiology,Lausanne University Hospital and University of Lausanne, 2Bioinformatics Core Facility,SIB Swiss Institute of Bioinformatics

Summary

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The role of RNA modifications in viral infections is just starting to be explored and could highlight new viral-host interaction mechanisms. In this work, we provide a pipeline to investigate m6A and m5C RNA modifications in the context of viral infections.

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M6AM5CEpitranscriptomeViral InfectionHIVRNA ExtractionMRNA IsolationPoly-A SelectionRNA-seq

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Cristinelli, S., Angelino, P., Ciuffi, A. Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV. J. Vis. Exp. (181), e62426, doi:10.3791/62426 (2022).

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