/

Biologie du développement

/

患者外周血单核细胞中人心肌细胞的生成和扩增

Journal JoVE
Biologie du développement

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Biologie du développement

Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Please note that all translations are automatically generated. Click here for the English version.

患者外周血单核细胞中人心肌细胞的生成和扩增

DOI:

10.3791/62206-v

•

05:38 min

•

February 12, 2021

•

Shiqiao Ye², Xiaoping Wan, Juan Su², Akshar Patel^2,4, Blake Justis^2,5, Isabelle Deschênes, Ming-Tao Zhao^2,5,6

¹Center for Cardiovascular Research, The Abigail Wexner Research Institute,Nationwide Children’s Hospital, ²The Heart Center,Nationwide Children’s Hospital, ³Department of Physiology and Cell Biology,The Ohio State University College of Medicine, ⁴Department of Anatomy,The Ohio State University College of Medicine, ⁵MCDB Graduate Program,The Ohio State University, ⁶Department of Pediatrics,The Ohio State University College of Medicine

Chapitres

00:04Introduction
01:08Human Induced Pluripotent Stem Cell (iPSC) Maintenance and Passaging
01:55Chemically Defined Cardiomyocyte (CM) Differentiation
02:57Results: Representative iPSC-CM Differentiation and Function
04:51Conclusion

Summary

Traduction automatique

在这里，我们提出了一种方案，可以从患者外周血单核细胞中稳健地产生和扩增人心肌细胞。

Tags

Keywords: Cardiomyocyte Generation Peripheral Blood Mononuclear Cells IPSC Reprogramming Cardiovascular Disease Modeling Cardiac Toxicity Testing Differentiation Protocol Cell Culture Sendai Virus PBMC E8 Medium Cardiomyocyte Differentiation Media

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

人类神经干细胞从外周血造血祖细胞直接诱导

从人外周T细胞无饲养条件下，使用仙台病毒诱导多能干细胞的生成

从人胎盘组织源分离和间充质干扩张/基质细胞

从人多能干细胞使用高度可重复的小分子为基础的分化协议心肌细胞规模化生产

从血细胞使用仙台病毒和离心诱导多能干细胞代

从人体外周血单个核细胞融合，无诱导多能干细胞的生成方法游离型载体

界定和由人多能干细胞的肝细胞样细胞的可扩展代

分化软骨细胞与外周血源性人诱导多能干细胞

人脐血内皮祖细胞的分离

人多潜能干细胞第一心场样心脏祖细胞和心室样细胞的生成

Read Article