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Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis
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Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis
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06:09 min
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February 02, 2021
DOI:
1Krembil Research Institute, Toronto Western Hospital,University Health Network, 2Department of Laboratory Medicine and Pathobiology,University of Toronto, 3Department of Surgery, Division of Neurosurgery,University of Toronto, 4Department of Medicine, Division of Neurology,University of Toronto, 5Department of Medicine, Division of Neurology, Edmond J. Safra Program in Parkinson’s Disease and the Morton and Gloria Shulman Movement Disorders Clinic, Toronto Western Hospital,University Health Network, 6Tanz Centre for Research in Neurodegenerative Diseases,University of Toronto
Summary
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Here we present an automated method for semi-quantitative determination of dopaminergic neuron number in the rat substantia nigra pars compacta.
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O'Hara, D. M., Kapadia, M., Ping, S., Kalia, S. K., Kalia, L. V. Semi-Quantitative Determination of Dopaminergic Neuron Density in the Substantia Nigra of Rodent Models using Automated Image Analysis. J. Vis. Exp. (168), e62062, doi:10.3791/62062 (2021).
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