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小鼠视网膜的经瞳双光子体内成像

Journal JoVE
Neurosciences

This content is Free Access.

Journal JoVE Neurosciences

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

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小鼠视网膜的经瞳双光子体内成像

DOI:

10.3791/61970-v

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09:03 min

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February 13, 2021

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Zelun Wang², Sean McCracken², Philip R. Williams^3,4

¹John F. Hardesty, MD Department of Ophthalmology and Visual Sciences,Washington University School of Medicine, ²Graduate Program in Neuroscience,Washington University School of Medicine, ³Department of Neuroscience,Washington University School of Medicine, ⁴Hope Center for Neurological Disorders,Washington University School of Medicine

Chapitres

00:05Introduction
00:42Imaging Preparation
03:44Two-Photon Image Acquisition
06:13Mouse Recovery
06:46Results: Representative In Vivo Mouse Retina Imaging
08:36Conclusion

Summary

Traduction automatique

体内成像是研究健康和疾病生物学的有力工具。该协议描述了使用标准双光子显微镜对小鼠视网膜进行经瞳孔成像。它还展示了不同的活体成像方法，以荧光标记视网膜的多个细胞队列。

Tags

Keywords: In Vivo Imaging Two-photon Microscopy Mouse Retina Ophthalmological Diseases Retinal Cells Stabilization Head Holder Pupil Dilation Ear Canal Pins Bite Bar Eye Lubricant Fluorescence Retinal Structures

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