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Immunologie et infection
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Feeding and Quantifying Animal-Derived Blood and Artificial Meals in Aedes aegypti Mosquitoes
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Feeding and Quantifying Animal-Derived Blood and Artificial Meals in Aedes aegypti Mosquitoes

Feeding and Quantifying Animal-Derived Blood and Artificial Meals in Aedes aegypti Mosquitoes

DOI:
10.3791/61835-v

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09:42 min

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October 22, 2020

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, , ,
1Laboratory of Neurogenetics and Behavior,The Rockefeller University, 2Department of Biological Sciences,Columbia University

Chapitres

  • 00:04Introduction
  • 00:49Meal Preparation
  • 02:22Meal Delivery to Mosquitoes
  • 04:32Quantification of Consumed Meals
  • 07:01Results: Glytube Blood-feeding with Fluorescein-based Quantification
  • 08:50Conclusion

Summary

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The goal of this protocol is to deliver animal-derived and artificial blood meals to Aedes aegypti mosquitoes through an artificial membrane feeder and precisely quantify the volume of meal ingested.

Tags

Aedes AegyptiMosquitoesBlood FeedingArtificial MealsFluorescence-based QuantificationMeal VolumeProtocolHigh-throughput MeasurementsProtein-free Saline MealsGLI Tube

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