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M1 和 M2 人类单细胞衍生细胞的极化和对 结核病 感染的流动细胞学分析
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection
DOI:

10:43 min

September 18, 2020

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Chapitres

  • 00:04Introduction
  • 00:44Peripheral Blood Mononuclear Cell (PBMC) Isolation
  • 01:40Monocyte-Derived Cell Differentiation and Polarization
  • 03:25Mycobacterium tuberculosis (Mtb) Culture
  • 05:00Monocyte-Derived Cell Mtb Infection
  • 06:03Flow Cytometry Staining and Analysis
  • 07:40Results: Representative Phenotypic M1 and M2 Cell Characterization Before and After Mtb Infection
  • 09:47Conclusion

Summary

Traduction automatique

该协议提供了一种方法来研究人类M1或M2极化巨噬细胞中的 分枝杆菌 感染,该方法基于外周血单核细胞与感染GFP标记的病毒株H37Rv的巨噬细胞的分化,并使用10色面板(包括选定的M1/M2标记的表达)用流动细胞学进行分析。

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