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Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
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Chimie
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Journal JoVE Chimie
Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

3,496 Views

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08:51 min

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September 15, 2020

DOI:

10.3791/61728-v

DOI:
10.3791/61728-v

•

08:51 min
September 15, 2020

•

6 Views
, , ,
1Centre for Biospectroscopy and School of Chemistry,Monash University, 2Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology,Monash University, 3Department of Infectious Diseases, The Alfred Hospital and Central Clinical School,Monash University

Summary

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Atomic Force Microscopy-Infrared Spectroscopy (AFM-IR) provides a powerful platform for bacterial studies, enabling to achieve nanoscale resolution. Both, mapping of subcellular changes (e.g., upon cell division) as well as comparative studies of chemical composition (e.g., arising from drug resistance) can be conducted at a single cell level in bacteria.

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Cite this Article

Kochan, K., Peleg, A. Y., Heraud, P., Wood, B. R. Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry. J. Vis. Exp. (163), e61728, doi:10.3791/61728 (2020).

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