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从高梁叶组织中分离出希石，用于自上而下质量光谱分析潜在的表观遗传标记
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07:10 min
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March 04, 2021
DOI:
1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute,Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center,University of California Agriculture and Natural Resources, 4West Side Research and Extension Center,University of California, 5Department of Plant Sciences,University of California, Davis, 6Department of Plant and Microbial Biology,University of California, Berkeley
Summary
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该方案的制定是为了有效地从高梁叶材料中提取完整的组织结石，用于分析结石转化后的修饰，这些修饰可以作为潜在的表观遗传标记，以帮助工程抗旱作物。
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Cite this Article
Zhou, M., Abdali, S. H., Dilworth, D., Liu, L., Cole, B., Malhan, N., Ahkami, A. H., Winkler, T. E., Hollingsworth, J., Sievert, J., Dahlberg, J., Hutmacher, R., Madera, M., Owiti, J. A., Hixson, K. K., Lemaux, P. G., Jansson, C., Paša-Tolić, L. Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers. J. Vis. Exp. (169), e61707, doi:10.3791/61707 (2021).
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