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Neuroscience

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JoVE Journal Neuroscience
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons
 

Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

Article DOI: 10.3791/61662-v • 06:21 min • October 6th, 2020
October 6th, 2020 •
*1,2, *1,3, 1
1Brudnick Neuropsychiatric Research Institute, Department of Neurobiology, University of Massachusetts Medical School, 2Interdisciplinary Graduate Program, University of Massachusetts Medical School, 3UMMS MD/PhD Program, University of Massachusetts Medical School
* These authors contributed equally

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Presented here is a protocol for single-cell electroporation that can deliver genes in both excitatory and inhibitory neurons across a range of in vitro hippocampal slice culture ages. Our approach provides precise and efficient expression of genes in individual cells, which can be used to examine cell-autonomous and intercellular functions.

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Single-Cell Electroporation Organotypic Slice Culture Mouse Hippocampal Excitatory Neurons Class-Specific Inhibitory Neurons Gene Transfection Neurobiology Studies Transfection Efficiency Cell Autonomous Mechanisms Transsynaptic Protein Interactions Slice Culture Medium Electroporation Rig Bleach Sterilization ACSF Tetrodotoxin
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