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使用单纳米粒子暗场显微镜可视化细胞膜上金纳米棒的扩散动力学
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Biochimie
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Journal JoVE Biochimie
Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy
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使用单纳米粒子暗场显微镜可视化细胞膜上金纳米棒的扩散动力学

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09:09 min

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March 05, 2021

DOI:

10.3791/61603-v

DOI:
10.3791/61603-v

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09:09 min
March 05, 2021

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3 Views
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1Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education),Tsinghua University

Summary

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在这里，我们展示了使用传统的暗场显微镜来监测金纳米棒（AuNR）在细胞膜上的动态。使用 ImageJ 和 MATLAB 检测单个 AuNR 的位置和方向，AuNR 的扩散状态以单个粒子跟踪分析为特征。

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Gold NanorodsSingle NanoparticleDarkfield MicroscopyCell MembraneDiffusion DynamicsTranslational DynamicsRotational DynamicsSingle Particle TrackingCTAB CoatingCell CultureImage ProcessingImageJ

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Ge, F., Xue, J., He, Y. Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy. J. Vis. Exp. (169), e61603, doi:10.3791/61603 (2021).

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