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A Patient-Derived Xenograft Model for Venous Malformation
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Biologie du développement
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Journal JoVE Biologie du développement
A Patient-Derived Xenograft Model for Venous Malformation

A Patient-Derived Xenograft Model for Venous Malformation

DOI:
10.3791/61501-v

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06:51 min

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June 15, 2020

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, ,
1Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine

Chapitres

  • 00:04Introduction
  • 00:42VM Patient-derived Xenograft Protocol
  • 03:11Tissue Collection and Processing
  • 03:45Analysis of Human-derived Vascular Channels
  • 05:13Results: VM Lesion Plug
  • 06:10Conclusion

Summary

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We present a detailed protocol to generate a murine xenograft model of venous malformation. This model is based on the subcutaneous injection of patient-derived endothelial cells containing hyper-activating TIE2 and/or PIK3CA gene mutations. Xenograft lesions closely recapitulate the histopathological features of VM patient tissue.

Tags

Patient-derived XenograftVenous MalformationPreclinical Therapeutic TestingEndothelial CellsVascular AnomaliesTrypsinizationCell SuspensionCentrifugationBMEMAnesthesiaInjectionCaliper MeasurementFormalin Fixation

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