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A Patient-Derived Xenograft Model for Venous Malformation
Capítulos
- 00:04Introduction
- 00:42VM Patient-derived Xenograft Protocol
- 03:11Tissue Collection and Processing
- 03:45Analysis of Human-derived Vascular Channels
- 05:13Results: VM Lesion Plug
- 06:10Conclusion
We present a detailed protocol to generate a murine xenograft model of venous malformation. This model is based on the subcutaneous injection of patient-derived endothelial cells containing hyper-activating TIE2 and/or PIK3CA gene mutations. Xenograft lesions closely recapitulate the histopathological features of VM patient tissue.
