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A Patient-Derived Xenograft Model for Venous Malformation
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JoVE Revista Biología del desarrollo
A Patient-Derived Xenograft Model for Venous Malformation

A Patient-Derived Xenograft Model for Venous Malformation

5,132 Views

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06:51 min

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June 15, 2020

DOI:

10.3791/61501-v

DOI:
10.3791/61501-v

•

06:51 min
June 15, 2020

•

2 Views
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1Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine

Summary

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We present a detailed protocol to generate a murine xenograft model of venous malformation. This model is based on the subcutaneous injection of patient-derived endothelial cells containing hyper-activating TIE2 and/or PIK3CA gene mutations. Xenograft lesions closely recapitulate the histopathological features of VM patient tissue.

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Schrenk, S., Goines, J., Boscolo, E. A Patient-Derived Xenograft Model for Venous Malformation. J. Vis. Exp. (160), e61501, doi:10.3791/61501 (2020).

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