Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University
Chapitres
- 00:04Introduction
- 00:33Sham and UUO Surgeries
- 01:52Collection of Kidney Samples
- 02:39Extraction of Total RNA from the Kidney Samples
- 05:28Synthesis of cDNA
- 06:29Results: Differentially Expressed miRNAs in the Kidneys of UUO Mice
- 07:08Conclusion
We describe a method for evaluating the microRNA expression in the kidneys of mice with unilateral ureteral obstruction (UUO) by quantitative reverse-transcription polymerase chain reaction. This protocol is suitable for studying kidney microRNA expression profiles in mice with UUO and in the context of other pathological conditions.
