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CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
Chapitres
- 00:04Introduction
- 01:04Protoplast Extraction
- 02:41Protoplast and PEG-Assisted Transformation and Transformant Recovery
- 04:27Phenotypic Mutant Strain Analysis
- 05:42Results: Representative Protoplast Extraction and Isolate Phenotype Analysis
- 06:45Conclusion
The CRISPR-Cas9 genome editing system is an easy-to-use genome editor that has been used in model and non-model species. Here we present a protein-based version of this system that was used to introduce a premature stop codon into a mating gene of a non-model filamentous ascomycete fungus.
