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Imaging and Analysis of Oil Red O-Stained Whole Aorta Lesions in an Aneurysm Hyperlipidemia Mouse Model
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Médecine
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Journal JoVE Médecine
Imaging and Analysis of Oil Red O-Stained Whole Aorta Lesions in an Aneurysm Hyperlipidemia Mouse Model

Imaging and Analysis of Oil Red O-Stained Whole Aorta Lesions in an Aneurysm Hyperlipidemia Mouse Model

DOI:
10.3791/61277-v

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07:57 min

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May 02, 2022

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, ,
1Yale Cardiovascular Research Center, Department of Internal Medicine,Yale University School of Medicine, 2Department of Surgery,Yale University School of Medicine, 3Department of Cell Biology,Yale University School of Medicine

Chapitres

  • 00:05Introduction
  • 00:38Heart Perfusion
  • 01:39Aorta and Aortic Branch Isolation
  • 02:36Heart and Aorta Fixation
  • 03:29Unopened Whole Aorta Oil Red O Staining and Imaging
  • 04:43En Face Aorta Mounting
  • 05:39Results: Representative Atherosclerotic Lesion Visualization and Quantification
  • 06:57Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol provides a step-by-step procedure to analyze atherosclerotic burden in mice. Investigators can use this protocol to compare the abundance, location, and size of atherosclerotic lesions in different animals.

Tags

Keywords: AortaAtherosclerosisAneurysmHyperlipidemiaMouse ModelOil Red O StainingPerfusionDissectionFixationQuantificationVascular Lesions

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