Journal
/
Biologie du développement
/
为早期小鼠胚胎测序准备小型RNA库
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
Please note that all translations are automatically generated. Click here for the English version.

为早期小鼠胚胎测序准备小型RNA库

5,035 Views

•

08:37 min

•

October 09, 2020

DOI:

10.3791/61086-v

DOI:
10.3791/61086-v

•

08:37 min
October 09, 2020

•

22 Views
,
1Development, Disease Models & Therapeutics Graduate Program, Department of Molecular and Cellular Biology, Department of Neuroscience, Center for Cell and Gene Therapy, Stem Cells and Regenerative Medicine Center,Baylor College of Medicine

Summary

Automatically generated

我们描述了一种在早期小鼠胚胎中分析微RNA的技术。该协议克服了低细胞输入和小RNA富集的挑战。此测定可用于分析早期小鼠胚胎不同细胞系中 miRNA 表达随时间变化。

Explore More Videos

Small RNA SequencingMicroRNAsEmbryonic DevelopmentLow Input SamplesEmbryo DissectionEmbryo StagingCell SuspensionCell SortingRNA Extraction

Read Article

Cite this Article

Keuls, R. A., Parchem, R. Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos. J. Vis. Exp. (164), e61086, doi:10.3791/61086 (2020).

Download .ris file

Copy

Share Video

.

Copy