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Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish
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Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish
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April 29, 2021
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This assay is a simple method to quantitate hematopoietic cells in developing embryonic zebrafish. Blood cells from dissociated zebrafish are subjected to flow cytometry analysis. This allows the detection of blood defects in mutant animals and after genetic modification.
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Rueb, K. F., Stachura, D. L. Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish. J. Vis. Exp. (170), e61035, doi:10.3791/61035 (2021).
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