Journal
/
Immunologie et infection
/
Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

4,140 Views

•

08:52 min

•

February 05, 2021

DOI:

10.3791/60949-v

DOI:
10.3791/60949-v

•

08:52 min
February 05, 2021

•

2 Views
, , ,
1Department of Pathology, Renaissance School of Medicine,Stony Brook University, Stony Brook, NY, 2Stony Brook Cancer Center, Stony Brook, NY, 3Department of Medicine,Weill Cornell Medicine, New York, NY

Summary

Automatically generated

Here, we provide a detailed description of the procedure to induce colonoscopic-guided pinch biopsies in mice and track wound closure in real time. Additionally, methods for the preparation of tissues for histological, immunohistochemical and molecular analyses of the wound bed are provided.

Explore More Videos

Colonoscopic-guided Pinch BiopsyMiceColonic Wound HealingInflammatory Bowel Disease (IBD) PathogenesisEndoscopeBiopsy ForcepsAnesthesiaPBSVideo Recording

Read Article

Cite this Article

Montrose, D. C., McNally, E. M., Sue, E., Dannenberg, A. J. Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes. J. Vis. Exp. (168), e60949, doi:10.3791/60949 (2021).

Download .ris file

Copy

Share Video

.

Copy