Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1The Department of Physiology and Cell Biology, Faculty of Health Sciences,Ben-Gurion University of the Negev, 2The Regenerative Medicine and Stem Cell (RMSC) Research Center,Ben-Gurion University of the Negev, 3The Zlotowski Center for Neuroscience,Ben-Gurion University of the Negev, 4The Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center, 5Division of Micro and Nanosystems,KTH Royal Institute of Technology, 6AIMES, Department of Neuroscience,Karolinska Institutet
Chapitres
- 00:05Introduction
- 01:27Seeding the “Brain Side” Channel and Differentiating EZ Spheres into Mixed Neural Cultures
- 03:04Seeding iBMECs (iPSC-Derived Brain Microvascular Endothelial Cells) into the Bottom Channel to Generate the “Blood Side”
- 05:27Initiation of Flow
- 08:12Results: Immunocytochemistry and Permeability of the iPSC-Base Blood-Brain Barrier Chip
- 09:17Conclusion
Blod-hjernebarrieren (BBB) er en flercellet nevrovaskulær enhet som regulerer hjernens homeostase tett. Ved å kombinere menneskelige iPSCer og organ-on-chip-teknologier, har vi generert en personlig BBB-chip, egnet for sykdomsmodellering og CNS-prognoser for legemiddelpenetrabilitet. En detaljert protokoll er beskrevet for generering og drift av BBB-chipen.
